Flag-conjugated proteins
A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody. denoted as M1. binds the peptide in the presence of bivalent metal cations. preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide.
| Cat No.： | PTAB1450013 |
| Product Name： | Flag-conjugated proteins |
| Type： | Protein |
| Property： | |
| Origin： | |
| Source： | mouse |
| Application： | |
| Formulation： | |
| Purity： | >95% by SDS-PAGE gel |
| Storage： | Avoid repeated freeze-thaw cycles. 12 months at -20 C to -80 C. 1 month at 2 C to 8 C. |
| Limitation： | |
| Note： |
