
Flag-conjugated proteins | |
Type | Mouse Antibody Monoclonal |
Introduction | A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody. denoted as M1. binds the peptide in the presence of bivalent metal cations. preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide. |
Clone | NYRFLAG |
Immunogen | Flag-conjugated proteins |
Ig Subclass | Mouse IgG1/kappa |
Purification Method | Protein A column. |
Usage | FOR LABORATORY RESEARCH USE ONLY. |
Applications | WB, IP, IF |
Cat No.: | PTAB1450013 |
Product Name: | Flag-conjugated proteins |
Type: | Protein |
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Application: | WB, IP, IF |
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